REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF PHOSPHOLIPASES: APPLICATION OF SPECTROPHOTOMETRIC DETECTION TO RAT PHOSPHOLIPASE A2 ISOZYMES

This paper appraises an HPLC method for assaying phospholipase A2 (PLA2).The procedure eco balloons is based on heptane-isopropanol-H2SO4 extraction of fatty acids released by the enzyme in the presence of margaric acid as an internal standard, and precolumn derivatization with 9-anthryldiazomethane.The derivatives of naturally occurring long-chain

Silicone rubber insulators are replacing the conventional ceramic and porcelain insulators rapidly in power transmission and distribution industry.Very limited field knowledge is available about the performance of silicone rubber insulators in polluted and contaminated environments and therefore need further investigation.A comprehensive analysis o

Prescribed burns and selective shrub clearing are widely implemented as management strategies to stop the shrub encroachment of grasslands, decrease fuel loads and fire risks, and improve biodiversity and ecosystem functionality in mountain environments.While the short-term effects of burns on soil have been extensively studied, the impact of mecha

The study of the effectiveness of economic diplomacy has become a popular topic in the field of international economic relations.Recent trends are confirmed by a large number of academic studies focusing on the evaluation of the performance of diplomatic and consular offices abroad.This paper represents a new attempt to empirically examine the effe

Background Managed populations of the alfalfa leafcutting bee (ALCB), Megachile rotundata (F.), are often not sustainable.In addition to numerous mortality factors that contribute to this, the dense bee populations used to maximize alfalfa pollination quickly deplete floral resources available to bees later in the summer.Providing alternative flora